Research Paper Volume 15, Issue 21 pp 12476—12496
lncRNA HOXC-AS2 promotes the progression of hypopharyngeal cancer by binding to the P62 protein mediating the autophagy process
Figure 6. HOXC-AS2 affected the expression of HMOX1 by regulating the NF-KB signaling pathway and ultimately regulated autophagy in hypopharyngeal cancer cells. (A) Transcriptome sequencing was performed after HOXC-AS2 knockdown, and the heatmap shows the differentially expressed genes identified by sequencing. NC-transfected cells were used as controls. (B) The mRNA expression level of HMOX1 was confirmed through qRT-PCR analysis after HOXC-AS2 overexpression. (C) The protein expression level of HMOX1 was confirmed through western blot analysis after HOXC-AS2 overexpression. (D) The mRNA expression level of HMOX1 was confirmed through qRT-PCR analysis after HOXC-AS2 knockdown. (E) The protein expression level of HMOX1 was confirmed through western blot analysis after HOXC-AS2 knockdown. (F, G) Autophagosome formation in hypopharyngeal cancer cells after HOXC-AS2 knockdown was observed by transmission electron microscopy, and NC-transfected cells were used as controls. (H) The protein expression level of HMOX1 was affected by HOXC-AS2 and NF-KB.