Research Paper Volume 15, Issue 21 pp 12476—12496
lncRNA HOXC-AS2 promotes the progression of hypopharyngeal cancer by binding to the P62 protein mediating the autophagy process
Figure 5. HOXC-AS2 activated the NF-kB signaling pathway by binding to the SQSTM 1 protein. (A) Representative image of P62 protein expression in normal hypopharyngeal carcinoma-adjacent tissue, as evaluated by IHC staining. (B) Representative image of P62 protein expression in hypopharyngeal carcinoma tissue, as evaluated by IHC staining. (C) The expression level of P62 in head and neck squamous cell carcinoma was confirmed by TCGA database analysis. (D) Activation of the NF-KB signaling pathway after HOXC-AS2 overexpression was detected by western blot analysis. (E) The protein expression level of NF-KB was measured by western blot analysis after P62 was knocked down in HOXC-AS2-overexpressing cells.