Research Paper Volume 15, Issue 21 pp 12476—12496
lncRNA HOXC-AS2 promotes the progression of hypopharyngeal cancer by binding to the P62 protein mediating the autophagy process
Figure 3. HOXC-AS2 enhanced the viability, invasion, proliferation, and migration of hypopharyngeal cancer cells in vitro (magnification: 200×). The data are shown as the means ± SEMs (n = 3); ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001. (A) The overexpression efficiency of LV-Oe-HOXC-AS2 and LV-OeNC-HOXC-AS2 in FADU cells was evaluated by qRT-PCR. (B) The knockdown efficiency of LV-KD-HOXC-AS2 and LV-KDNC-HOXC-AS2 in Detroit-562 cells was evaluated by qRT-PCR. (C) The viability of stable LV-Oe-HOXC-AS2-FADU and LV-OeNC-HOXC-AS2-FADU cell lines was evaluated by a CCK-8 assay. (D) The viability of LV-SH-HOXC-AS2-Detroit-562 and LV-SHNC-HOXC-AS2-Detroit-562 cells was evaluated by a CCK-8 assay. (E) The invasion ability of LV-Oe-HOXC-AS2-FADU and LV-OeNC-HOXC-AS2-FADU stable cells was evaluated by a Transwell assay. (F) The migration ability of LV-Oe-HOXC-AS2-FADU and LV-OeNC-HOXC-AS2-FADU stable cells was evaluated by a Transwell assay. (G) The migration ability of LV-Oe-HOXC-AS2-FADU and LV-OeNC-HOXC-AS2-FADU stable cells was evaluated by a wound healing assay. (H) The invasion ability of LV-SH-HOXC-AS2-Detroit-562 and LV-SHNC-HOXC-AS2-Detroit-562 stable cells was evaluated by a Transwell assay. (I) The migration ability of LV-KD-HOXC-AS2-Detroit-562 and LV-KDNC-HOXC-AS2-Detroit-562 stable cells was evaluated by a Transwell assay. (J) The migration ability of LV-SH-HOXC-AS2-Detroit-562 and LV-SHNC-HOXC-AS2-Detroit-562 stable cells was evaluated by a wound healing assay.