Priority Research Paper Volume 15, Issue 20 pp 10821—10855

Figure 5. Native and ECM-enriched ovaries have distinct age-associated proteomic signatures. (A, B) Partial least squares-discriminant analysis performed with quantifiable proteins from native (A, N = 4,084) and ECM-enriched (B, N = 3,160) ovaries shows distinct, age-dependent clustering in both native and ECM-enriched conditions. (C, D) Volcano plots of significantly altered proteins comparing (C) old vs. young native ovaries (N = 180) and (D) old vs. young ECM-enriched ovaries (N = 279). In native ovaries, 103 proteins are significantly upregulated and 77 proteins are significantly downregulated with age. In ECM-enriched ovaries, 125 proteins are significantly upregulated and 154 proteins are significantly downregulated with age. Filtering criteria to determine significantly altered proteins are as follows: q-value < 0.01 and |log₂(Old/Young)| > 0.58.