Priority Research Paper Volume 15, Issue 20 pp 10821—10855

Figure 3. ECM-enrichment was effective in ovaries from reproductively young and reproductively old mice. (A) Representative scans of ovarian tissue sections from reproductively young and old mice stained with H&E following treatment with 0.1% SDS for 12.5 h. Example of a follicle indicated by Ø and example of a corpus luteum indicated by ‡. (B) Quantification of relative ECM-enriched area (%). (C, D) Representative images of Hematoxylin (C) and Hoechst (D) stained ovarian tissue sections from reproductively young and old mice following treatment with 0.1% SDS for 0 h (Native) or 12.5 h (ECM-enriched). Bottom row of each panel is optical zoom of boxed region from top row. Scale bar for top row for each panel = 400 μm. Scale bar for bottom row for each panel = 100 μm. (E, F) Images of agarose gel electrophoresis of DNA extracted from native (N) and ECM-enriched (E) ovaries from reproductively young (E) and old (F) mice. Numbers (ex: 1N and 1E) correspond to ovaries from the same mouse. Samples run with 1 kb DNA ladder. N = 4–5 ovaries per group.