Research Paper Volume 15, Issue 21 pp 12369—12387

Figure 7. CCL19 modulates tumor immune microenvironment features and predicts prognosis in breast cancer patients. (A) We implemented with the CIBERSORT algorithms and identified prominently positive association between CCL19 expression and tumor-associated immune cells, including B cells, CD4+ T cells, CD8+ T cells, neutrophil, and myeloid dendritic cells. (B) Then, Sperman’s correlations between CCL19 expression and the immune cells infiltration were shown in the form of dot plots. (C) Multiplex fluorescence was implemented to identify Treg, CD8⁺ T cells, and PD-L1⁺ macrophages. (D) IHC staining of CCL19 in a total of 182 patients with breast cancer. (E) Differential percent of specific cells were compared between CCL19 expression groups using Student’s t test. (F) Kaplan-Meier survival analysis revealed that higher expression of CCL19 significantly predicts long-term outcomes for breast cancer patients.