Research Paper Volume 15, Issue 22 pp 12723—12737
Tat-heat shock protein 10 ameliorates age-related phenotypes by facilitating neuronal plasticity and reducing age-related genes in the hippocampus
Figure 4. Effects of Tat-HSP10 and HSP10 on the expression of aging-related genes and synaptic plasticity-related proteins in the hippocampus. (A) Real-time PCR is performed to detect Sirt1, Sirt2, and FoxO1 mRNA levels in the control, HSP10-, and Tat-HSP10-treated groups of adult and aged mice. In addition, (B) NMDAR1, PSD95, and VGLUT2 protein levels are detected using western blotting in aged mice. Data from western blotting are quantified based on the immunodensity of each band. Data are represented as the mean ± SD (n = 5 each group; analyzed by two-way ANOVA test followed by Tukey’s post hoc test, aP < 0.05, vs. adult group; bP < 0.05, vs. control group; cP < 0.05, vs. HSP10-treated group). Abbreviations: HSP: Heat shock protein; ANOVA: Analysis of Variance; SD: standard deviation; Sirt1: sirtuin 1; Sirt2: sirtuin 2; FoxO1: forkhead box O1; NMDAR1: N-methyl-D-aspartate receptor 1; PSD95: postsynaptic density 95; VGLUT2: vesicular glutamate transport 2.