Research Paper Volume 15, Issue 22 pp 12723—12737
Tat-heat shock protein 10 ameliorates age-related phenotypes by facilitating neuronal plasticity and reducing age-related genes in the hippocampus
Figure 3. Effects of Tat-HSP10 and HSP10 on cell proliferation and neuroblast differentiation in adult and aged mice. Immunohistochemical staining for (A) Ki67 and (B) DCX are conducted to visualize the proliferating cells and differentiated neuroblasts in the dentate gyrus, respectively, in the control, HSP10-, and Tat-HSP10-treated groups of adult and aged mice. Scale bar = 50 μm. Immunohistochemical staining is quantified by counting the Ki67-immunoreactive nuclei in the subgranular zone and measuring the immunodensity of DCX-immunoreactive neuroblasts in the whole dentate gyrus. The immunodensity of DCX is normalized into percentile value vs. the control group of adult mice. Data are represented as the mean ± SD (n = 5 each group; analyzed by two-way ANOVA test followed by Tukey’s post hoc test, aP < 0.05, vs. adult group; bP < 0.05, vs. control group; cP < 0.05, vs. HSP10-treated group). Abbreviations: HSP: Heat shock protein; ANOVA: Analysis of Variance; SD: standard deviation; DCX: doublecortin.