Research Paper Volume 15, Issue 21 pp 12251—12263
Polyphyllin I attenuates the invasion and metastasis via downregulating GRP78 in drug-resistant hepatocellular carcinoma cells
Figure 5. PPI exerts a blockade on the invasion and metastasis of SR-HCC cells by suppressing GRP78. (A) An ensemble of PPI monomer structures was determined in molecular docking calculations. (B, C) The results obtained from molecular docking software (AutoDock Vina) are presented. (D) Western blot analysis was performed to detect the expression of various proteins in HepG2/S rGRP78 cells. (E) Immunofluorescence experiments were conducted to examine the expression of GRP78, E-cadherin, Vimentin, and ABCG2 in HepG2/S rGRP78 cells that had been treated with PPI. Statistical significance is indicated by asterisks (*P < 0.05, **P < 0.01, ***P < 0.001).