Research Paper Volume 15, Issue 21 pp 12155—12170

Figure 7. Overexpression of circ.5863 through lentiviral transfection can alleviate inflammation in IC/BPS. (A) qPCR validation of overexpression of circ.5863. (B–F) The qPCR results from relative quantitative analysis showed that overexpression of circ.5863 can restore barrier function and reduce the expression levels of IL-1β, and TNF-α. (G–J) The Western blot results and relative protein quantification analysis showed that overexpression of circ.5863 can reduce the levels of IL-1β, IL-6, and TNF-α. Results were presented as mean ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. All of the experiments were performed in triplicate. “coecon” refers to a negative control of HUCs transfected with lentivirus overexpression. “Coeconl” refers to the LPS-induced IC/BPS model of the negative control. “Coe” refers to the overexpression of Circ.5863, while “Coel” refers to the LPS-induced IC/BPS model after overexpression of Circ.5863.