Research Paper Volume 15, Issue 21 pp 12120—12135

Figure 6. Overexpressing miR-124-3p reversed USP14-mediated oncogenic function. (A) miR-124-3p mimics or miR-NC were transfected into USP14-overexpressing HEC-1-B cells, and qRT-PCR was used to check the miR-124-3p profile. (B) The colony formation experiment verified the colony formation of HEC-1-B and SNG-M cells. (C) FCM was implemented to verify EC apoptosis. (D) Bcl-2, Bax, and Cyclin D1 protein profiles were compared by WB. (E) Transwell assays were used to test EC cell migration and invasion. (F) The levels of E-cadherin, Vimentin, Snail1, ZEB1, and Slug were tested by WB. ^**, ^***indicate P < 0.01 and P < 0.001, vs. USP14+miR-NC. N = 3.