Research Paper Volume 15, Issue 21 pp 12120—12135

Figure 4. The effects of USP14 knockdown in the apoptosis, migration and invasion of EC cells. (A) sh-USP14 or sh-NC was transfected into HUC-1-B and SNG-M cells, and the USP14 profile in EC cell lines was examined by WB. (B) Colony formation of HEC-1-B and SNG-M cells was tracked by colony formation experiments. (C) FCM was used to observe apoptosis. (D) The expression levels of Bcl-2, Bax, and Cyclin D1 were examined by WB. (E) Transwell assays were implemented to evaluate EC cell migration and invasion. (F) E-cadherin, Vimentin, Snail1, ZEB1, and Slug profiles were examined by WB. NS, ^*, ^**, ^***indicate P > 0.05, P < 0.05, P < 0.01, and P < 0.001, respectively, vs. sh-NC. N = 3.