Research Paper Volume 15, Issue 21 pp 12104—12119
Collaborating single-cell and bulk RNA sequencing for comprehensive characterization of the intratumor heterogeneity and prognostic model development for bladder cancer
Figure 4. Subclassification analysis of fibroblasts and endothelial cells. (A) Ten clusters of fibroblasts were shown in the form of a UMAP diagram. (B) The distribution of myofibroblasts and inflammatory fibroblasts in the whole fibroblast population. (C) The bubble plot showed the cell communication analysis results of iCAFs with different cell types. (D) The bubble plot showed the cell communication analysis results of myo-CAFs with different cell types. (E) The myofibroblast markers (αSMA, TAGLN, MYLK, MYL9) and iCAFs-specific inflammatory marker genes (CFD, MFAP5, DCN) were shown in the form of bubble map. (F) Subclustering of the endothelial cells. (G) The heat map showed the enriched transcription factors of various endothelial cell types. (H) The transcriptional activity of SRF in different cell types was shown on the UAMP plot. (I) The transcriptional activity of ERG in different cell types was shown on the UAMP plot.