Research Paper Volume 15, Issue 21 pp 11970—11984
Identification of epithelial-mesenchymal transition-related biomarkers in lung adenocarcinoma using bioinformatics and lab experiments
Figure 7. Silencing of PLEK2 prohibits the autophagy of LUAD cells. (A) The protein levels of LC3II/I, ATG5 and P62 in A549 cells after transfection of si-PLEK2#1, si-PLEK2#2 or si-NC were determined by western blotting (P < 0.01), suggesting silencing of PLEK2 could critically inhibit the autophagy of LUAD cells. (B) The protein levels of LC3II/I, ATG5 and P62 in NCI-H1299 cells after transfection of si-PLEK2#1, si-PLEK2#2 or si-NC were determined by western blotting (P < 0.01), suggesting silencing of PLEK2 could critically inhibit the autophagy of LUAD cells.