Research Paper Volume 15, Issue 21 pp 11970—11984
Identification of epithelial-mesenchymal transition-related biomarkers in lung adenocarcinoma using bioinformatics and lab experiments
Figure 6. Silencing of PLEK2 reduces the proliferative and migration ability of LUAD cells. (A) The expression of PLEK2 after transfection of si-PLEK2#1, si-PLEK2#2 or si-NC in A549 and NCI-H1299 cells was detected via RT-PCR (P < 0.01), demonstrating that PLEK2 was successfully knocked down in A549 and NCI-H1299 cells. (B) The viability of A549 and NCI-H1299 cells after transfection of si-PLEK2#1, si-PLEK2#2 or si-NC was assessed by CCK-8 assay (P < 0.05), demonstrating that decreased PLEK2 could lead to a reduction in the proliferative ability in A549 and NCI-H1299 cells. (C) The migration of A549 and NCI-H1299 cells after transfection of si-PLEK2#1, si-PLEK2#2 or si-NC was calculated through wound healing assay (P < 0.01), demonstrating that decreased PLEK2 could lead to a reduction in the migration ability in A549 and NCI-H1299 cells.