Figure 9. M1 macrophage migration is mediated by XCL2. (A–C) The transduction outcomes of XCL2 in 786O, MDA-MB-231, and U251 are confirmed using western blotting. (D) The M1 macrophages induction procedure and morphology. (E) The schematic design shows the coculture of 786O, MDA-MB-231, or U251 cells with M1 macrophages. (F–I) The movement of M1 macrophages in coculture with 786O, MDA-MB-231, and U251 cells that have been XCL2-OE and XCL-NC transfected. The magnification under the microscope is shown as marked in the figure. *P < 0.05, **P < 0.01, and ***P < 0.001.