Research Paper Volume 15, Issue 21 pp 11875—11890
MicroRNA-30a-3p: a potential noncoding RNA target for the treatment of arteriosclerosis obliterans
Figure 5. ROCK2 attenuated the microRNA-30a-3p inhibitory effect. (A, B) A lentivirus vector (LV-ROCK2) upregulated ROCK2 in VSMCs (WB, n=3). (C, D) LV-ROCK2 infection recovered ROCK2 expression levels in microRNA-30a-3p-transfected VSMCs (WB, n=6). (E, F) LV-ROCK2 promoted VSMC proliferation and attenuated the inhibition of VSMC proliferation by microRNA-30a-3p (EdU assay, n=6). (G, H) LV-ROCK2 promoted VSMC migration and attenuated the microRNA-30a-3p inhibitory effect on VSMC migration (Transwell, n=6). Scale, 100 μm (E, G); *=0.05, **=0.005, ***=0.001.