Research Paper Volume 15, Issue 20 pp 11639—11653

Figure 4. Alisol A inhibited the expression of astrocytes, microglia, IL-6 and IL-1β after CI. (A) Representative image of GFAP in the hippocampal CA1 region by immunofluorescence staining. (B) Representative image of Iba1 in the hippocampal CA1 region by using immunofluorescence staining. 200×, scale bar is 100 μm. (C) Quantitative analysis of GFAP fluorescence intensity. (D) Quantitative analysis of Iba1 fluorescence intensity, n = 3. (E) Western blot showing the expression of IL-6 and quantitative analysis of the ratio of IL-6 to GAPDH. (F) Western blot showing the expression of IL-1β and quantitative analysis of the ratio of IL-1β to GAPDH, n = 4. Data are shown as the mean ± SD. ^*P < 0.05, ^**P < 0.01 compared with the sham group, ^#P < 0.05 compared with the CI group, ^@P < 0.05, ^@@P < 0.01 compared with the CI+AA group.