Research Paper Volume 15, Issue 20 pp 11639—11653

Figure 3. Alisol A protected the neurovascular ultrastructure in the hippocampal CA1 region. (A) Changes in neuronal ultrastructure are indicated by black arrows. (a) 1: The nuclear membrane structure of the neurons was intact and clear; 2: Chromatin distributed evenly; 3: Normal structural organelles were abundant within the cytoplasm. (b) 1: Incoherent nuclear membrane structure; 2: Chromatin condensation, decreased and edge aggregation; 3: Most of the organelles disappeared, and the cytoplasm was empty. (c) 1: The nuclear membrane is relatively complete, and the edge is clear; 2: More normal chromatin; 3: The organelles can be seen in the cytoplasm. (d) 1: Discontinuous and indistinct nuclear membrane structure; 2: Less chromatin; 3: Partial dissolution and disappearance of intracytoplasmic organelles. (a–d) Magnification of the microphotograph is 2500X. Scale bar is 5 μm; (e–h) Magnifications of the microphotograph are 8000X. Scale bar is 1 μm. (B) Changes in BBB ultrastructure as indicated by the red arrow. (a) 1: Clear capillary structures; 2: Close contact of the endothelial cells with the basement membrane; 3: Continuous endothelial cells; 4: End-feet of astrocyte surrounding the basal membrane. (b) 1: Capillary structure enhancement; 2: Edema fluid between the endothelial cells and the basement membrane; 3: Discontinuity of endothelium. 4: Marked edema of astrocytic end-foot processes and vacuolization. (c) 1: The edema between endothelial cells and the basal layer was weakened; 2: The endothelium was more continuous; 3: Vacuolization of astrocytic end-foot process edema was alleviated. (d) 1: There is edema between the endothelial cells and the basement membrane; 2: The endothelium is discontinuous and interrupted; 3: Perivascular astrocytic end-foot process edema was obvious and still showed vacuolar changes. (a–d) Magnification of the microphotograph is 2500X. Scale bar is 5 μm; (e–h) Magnifications of the microphotograph are 8000X. Scale bar is 1 μm. n = 3.