Research Paper Volume 15, Issue 20 pp 11611—11638
BCL2A1 is associated with tumor-associated macrophages and unfavorable prognosis in human gliomas
Figure 11. Knockdown of BCL2A1 inhibited GBM cell proliferation and macrophage migration. (A, B) Western blot analysis of the knockdown effect of BCL2A1 in U87 and U251 cells. (C) The effect of BCL2A1 knockdown on proliferation of GBM cells was detected by CCK8. (D, E) Edu assay indicated that BCL2A1 knockdown inhibited proliferation of GBM cells. (F) Diagram of co-culture of macrophages and GBM cells. (G, H) Knockdown of BCL2A1 in GBM cells inhibited the migration ability of co-cultured macrophages. (I) HE staining analysis. (J) Analysis of Kaplan-Meier survival in mice. (K) IHC analysis of BCL2A1, Ki67, CD68 and CCL2. ns: non-significant; *P < 0.05, **P < 0.01. Abbreviations: Nc: control group; Sh: BCL2A1 knockdown group.