Research Paper Volume 15, Issue 22 pp 12794—12816
Identification of mitochondrial-related signature and molecular subtype for the prognosis of osteosarcoma
Figure 9. The effect of MLYCD on osteosarcoma cell proliferation, migration, and invasion. (A) Protein expression levels of MLYCD were measured by western blotting. (B–D) CCK-8 and colony-formation assays were used to assess osteosarcoma cell proliferation. (E, F) The wound healing assay was performed to estimate the effect of MLYCD overexpression on cell migration. (G–J) The transwell assay was conducted to assess the effect of MLYCD overexpression on osteosarcoma cell invasion and migration. Scale bar = 100um. *P<0.05, **P<0.01, and ***P<0.001.