Research Paper Volume 15, Issue 20 pp 11471—11488
The Wnt/β-catenin signaling pathway inhibits osteoporosis by regulating the expression of TERT: an in vivo and in vitro study
Figure 8. The Wnt/β-catenin pathway promotes osteogenic differentiation and inhibits the apoptosis of BMSCs through upregulating TERT expression. (A–F) Western blotting and quantification of TERT, OPN, BMP2, Bcl-2, and BAX in NC, siGSK3β, siTERT, and siGSK3β + siTERT groups of BMSCs. (G, H) The Hoechst 33342/PI and quantification results in four groups of BMSCs. (I) ALP activity assay kit to detect ALP activity in four groups of BMSCs. Scale bar: 100 μm. All results were performed as mean ± SD. #P < 0.05 vs. NC group, *P < 0.05 vs. siGSK3β group. n = 6.