Research Paper Volume 15, Issue 20 pp 11448—11470

Figure 2. Ppih mRNA expression is significantly upregulated in Hepatocellular carcinoma (HCC) compared with normal tissues. (A) Expression of Ppih in HCC tissues (TCGA) and healthy liver tissues (TCGA) by RNA-Seq. (B) Paired expression data of Ppih mRNA in HCC and adjacent normal tissues by RNA-Seq in TCGA dataset. (C–F) Boxplot showing Ppih mRNA expression in liver (left plot) and HCC tissue (right plot) was derived from the Oncomine database. The fold-change in Ppih expression in HCC was determined using the Oncomine database. The data are HCC relative to normal liver tissue. The threshold was designated using the following specific parameters: p value = 1E-4, fold change = 2, and gene rank 10%. (G, H) Validation of the expression level of Ppih in HCC and normal tissues. GSE14520 and GSE76427 were regarded as the validation set. (I) Expression levels of Ppih mRNA in HBV-related HCC and paired nontumor liver tissues were investigated using RNA-seq (n = 158). These results were obtained from the studies of Gao et al. [17]. (J) mRNA expression of Ppih in cholangiocarcinoma (CHOL) tissues and adjacent normal tissues from the GEPIA 2 database. ^*p < 0.05.