Research Paper Volume 15, Issue 20 pp 11184—11200

Figure 6. Effect of miRNA-200a-3p on H₂O₂-induced pyroptosis of HAECs. (A) HAECs were treated with H₂O₂ (0.4 mM) for 1 h, 2 h, 4 h, and 8 h; miRNA-200a-3p expression increased in all cases compared with that in control cells. (B) qRT-PCR results showing the decreased expression of miR-200a-3p in HAECs transduced with the miR-200a-3p inhibitor compared with negative control cells. (C) LDH release from HAECs treated with H₂O₂ and transduced with or without the miR-200a-3p inhibitor. (D) PI staining results showing the decreased percentage of PI positive cells in HAECs transduced with the miR-200a-3p inhibitor. (E) Western blot demonstrating increased ratio of NLRP3, GSDMD-N, C-caspase-1, ASC, IL-1β, IL-18 in cells treated with H₂O₂ compared with cells that were not treated with H₂O₂. The ratio of NLRP3, GSDMD-N, C-caspase-1, ASC, IL-1β, IL-18 decreased in cells transduced with the miR-200a-3p inhibitor under H₂O₂ treatment compared with cells transduced with the NC inhibitor. (^*p<0.05 vs control, ^**p<0.01 vs control or NC, ^***p<0.001 vs control or NC, ^#p<0.05 vs H₂O₂, ^##p<0.01 vs H₂O₂)_. Data are presented as mean ± SD (n=3). HAECs, human aortic endothelial cells; qRT-PCR, quantitative real-time polymerase chain reaction; LDH, lactate dehydrogenase; PI, propidium iodide; NC, negative control.