Research Paper Volume 15, Issue 19 pp 10767—10784
Modified iPOND revealed the role of mutant p53 in promoting helicase function and telomere maintenance
Figure 5. The modified iPOND assay revealed that the p53S promoted the DNA replication through the recruitment of helicases to the replication forks. (A) The resolution of high molecular weight proteins was not good with original iPOND assay. (B) The resolution of high molecular weight proteins was improved by the modified iPOND assay. (C–F) The DNA helicase and DNA fork binding proteins revealed by modified iPOND assay. (C) The control without EdU incorporation. (D) EdU incorporated for 10 min. (E) EdU incorporated for 10 min, followed by thymidine chase for 1h. (F) EdU incorporated for 30 min.