Research Paper Volume 15, Issue 19 pp 10767—10784
Modified iPOND revealed the role of mutant p53 in promoting helicase function and telomere maintenance
Figure 2. The p53S reduced the presence of G4 structure by promoting the DNA helicase expression and colocalization with G4. (A) Detected by immunofluorescence with G4 antibody, the presence of G4 structure decreased in p53S cells comparing with p53null and WT cells, suggesting a smoother unwinding of G4 structure. (B) Detected by specific G4 binding protein G4P labeled with GFP, the presence of G4 structure decreased in p53S cells comparing with p53null and WT cells. (C) The comparison of the G4 content between p53S and p53H cells by immunofluorescence with G4 antibody. (D) The comparison of the G4 content between p53S and p53H cells by specific G4 binding protein G4P. (E) The p53S promoted the expression and colocalization of DNA helicases with G4 structure.