Research Paper Volume 15, Issue 19 pp 10767—10784

Figure 1. The p53S up-regulated the expression of DNA helicases and DNA fork protection complex proteins. (A) The schematic plot of the ChIP-chip assay and ssGSEA heatmap for p53S regulated pathways. The DNA replication pathways and DNA helicase pathways were up-regulated by p53S. (B) The binding of p53S to Mcm2 gene promoter was confirmed by the ChIP assay and semi-quantitative PCR. Both wild type p53 (left) and p53S (right) could bind to Mcm2 gene promoters with a little more abundance in p53S group. (C, D) The GSEA analysis of transcriptional enrichment in p53S cells comparing with WT cells. The enrichment plot displayed good enrichment signal in DNA replication pathways (C), the specific enrichment of individual genes is shown in (D). (E) The confirmation of protein expression regulation revealed by GSEA analysis. The DNA helicases, as well as telomere related proteins were up-regulated in p53S, comparing with p53null and WT cells. (F) The quantification of protein expression level in (E).