Research Paper Volume 15, Issue 19 pp 10746—10766

Figure 6. HOXD3 down-expression induces proliferation in ccRCC cells. (A, B) 786-O and CAKI-1 cells were transfected with siHOXD3-Ctrl, siHOXD3-1, and siHOXD3-2, and the HOXD3 expression at mRNA and protein levels was assessed by qRT-PCR and western blotting. (C) The effects of siHOXD3 on 786-0 and CAKI-1 cells viability were identified by MTT assay after transfecting of siHOXD3-Ctrl, siHOXD3-1 and siHOXD3-2. (D) Representative results of colony formation of 786-O and CAKI-1 cells after transfection of siHOXD3-Ctrl, siHOXD3-1, and siHOXD3-2. (E) Cell cycles were determined in 786-0 and CAKI-1 cells after transfection of siHOXD3-Ctrl, siHOXD3-1, and siHOXD3-2. (F, G) The wound healing and transwell assays were used to confirm the migration and invasion ability of siHOXD3 and control-transfected human ccRCC. Scale bar, 100 μm. (H) The expression of N-cadherin, E-Cadherin, MMP2, CDK4, and CyclinD1 was demonstrated by western blotting in both 786-O and CAKI-1 cells of HOXD3 down-expression (p < 0.01).