Priority Research Paper Volume 15, Issue 18 pp 9238—9249

Figure 1. Characteristic of X-irradiated corneal and conjunctival epithelial cells. (A) SA-β-gal expression in irradiated (IR) or non-irradiated (MOCK) CoEpiCs and CjEpiCs. (B) Percentage of SA-β-gal positivity (n = 4). (C) EdU incorporation in irradiated or non-irradiated CoEpiCs and CjEpiCs. (D) Percentage of EdU positivity (n = 3). (E) RNA expression analysis of p16 and p21 in irradiated or non-irradiated CoEpiCs (n = 3). (F) RNA expression analysis of p16 and p21 in irradiated or non-irradiated CjEpiCs (n = 3). (G) Protein expression analysis of p16 in irradiated or non-irradiated CoEpiCs (n = 3). (H) Protein expression analysis of p16 in irradiated or non-irradiated CjEpiCs (n = 3). Gene expression was normalized to the housekeeping gene GAPDH. ^*p < 0.05; ^**p < 0.01; ^***p < 0.001. Scale bars indicate 100 μm.