Research Paper Volume 15, Issue 19 pp 9984—10009

Figure 4. BMAL1 contributes to AP-1 mediated transcriptional control of the senescence program and confers resistance to apoptosis. (A) PCA plot of control and senescent WT and KO RNA-seq. (B, C) Volcano plot (B) and heatmap (C) of DEGs from WT and KO RNA-seq. (D, E) Venn diagrams of DEGs up- (D) and down- (E) regulated in WTS or KOS cells. (F, G) Gene ontology of pathways up- and downregulated in both WT and KO cells (F), and only in KO cells (G). (H, I) Heatmap of DEGs which are also AP-1 target genes upregulated (H) or downregulated (I) in KOS cells. (J) Confluence in senescent WT and KO cells after incubation with 0.156 μM, 0.313 μM, 0.625 μM, 1.25 μM and 2.5 μM ABT-263. Data is presented with mean +/− SEM; two-way ANOVA test with Sidak’s post hoc test for multiple comparisons were used (J) with significance indicated as ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001; n = 3 replicates for RNA-seq. n = 16 replicate fields for WTS confluence and n = 12 replicate fields for KOS confluence.