Research Paper Volume 15, Issue 19 pp 10732—10745
Micheliolide prevents estrogen deficiency-induced bone loss via inhibiting osteoclast bone resorption
Figure 6. The p38 agonist partly rescued the inhibitory effect of Micheliolide on osteoclastogenesis. BMMs were isolated, treated with “DMSO” or “MCL” (20 uM) or “Micheliolide and P79350” in each group together with M-CSF and RNAKL for 5 days. (A) Representative TRAP staining pictures. (B, C) Number and area of osteoclasts from each group were measured and quantified. (D) WB for P-P38, P38 and GAPDH was performed. All data: mean ± SD, n=5, **p < 0.01 comparing to DMSO group, ***p < 0.001 comparing to DMSO group, ##p < 0.01 comparing to MCL group, ###p < 0.001 comparing to MCL group.