Research Paper Volume 15, Issue 19 pp 10732—10745

Figure 4. Micheliolide inhibited osteoclast bone resorption and related gene expressions. (A) Representative images of bone resorption pits. (B) Bone resorption area (brown area) was measured. (C–G) mRNA expression of C-fos, Nfatc-1, CtsK, Trap and Dc-Stamp of mice BMMs received treatment of indicated doses of Micheliolide (5 ug/ml, 10 ug/ml, 20 ug/ml) or vehicle in the presence of M-CSF and RANKL for 5 days. All data: mean ± SD, n=5, *p < 0.05 comparing to Sham group, **p < 0.01 comparing to DMSO group.