Research Paper Volume 15, Issue 20 pp 11131—11151

Figure 3. Triptolide inhibits SAG-induced EOC cells proliferation, migration, and invasion. (A, B) TPL inhibited the cell proliferation provoked by SAG (1 μM). (C) Migration assay showed that TPL restrains the SAG-induced migration ability of SKOV3, A2780, and Ovcar8 cells. (D) Invasion assay showed that TPL inhibits SAG-induced invasion ability. (E) The quantification of EdU-positive cells was analyzed. Graph (F) represents the positive cell's number of migrations. Graphs (G) represent the positive cell's number of invasions. (H, I) The effects of SAG on the protein levels of MMP9, E-cadherin, and N-cadherin protein, and TPL could reverse the results produced by SAG, and GAPDH was used for normalization. Scale bar: 100 μm. All results were derived from three independent repeated experiments and represented by mean ± SD, * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. control; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. SAG group; ns, no significance.