Research Paper Volume 15, Issue 20 pp 10915—10937

The Saccharomyces cerevisiae acetyltransferase Gcn5 exerts antagonistic pleiotropic effects on chronological ageing

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Figure 1. Gcn5 exhibits an epistatic relationship with Hda1 in starvation-induced HSP expression and cell growth. (A, B) Starvation-induced expression levels of pHSP26-VFP (A) and pSSA3-VFP (B); (C) Relative growth rates. YPD (2% glucose) and YPGE (3% glycerol and 1% ethanol). The levels of the HSP reporters in WT cells at exponential phase (5.5h) were set to 1. The significance of differences was revealed by two-factor ANOVA analysis for HSP reporters across all the timepoints (A, B) or by student’s t-test for growth rates (C). ***: p < 0.001, **: 0.001<p< 0.01, *: 0.01<p< 0.05 and n.s.: p> 0.05. Error bars represent standard deviation calculated from biological triplicates.