Research Paper Volume 15, Issue 19 pp 10705—10731

Figure 8. Silencing of Glra2 alleviates visual acuity and retinal function. (A) Visual acuity was detected by the Cerebral Mechanics OptoMotry virtual-reality optokinetic tracking system. A simple staircase method was employed to detect the highest level of spatial frequency visible to the mouse. All tests were conducted at maximum contrast with a drift speed of 12°/s, starting at a spatial frequency of 0.042 cyc/deg. (B–D) ERG analysis was performed according to the standard practice and used a 60 Hz low pass digital filter to eliminate the contaminating noises from OPs. The amplitude of a-wave was measured from the baseline to the most negative trough, whereas b-wave amplitude was measured from the trough of a-wave to the highest positive peak. The representative waves are shown in Figure 8B. The amplitudes of a-wave and b-wave were statistically calculated. ^*P < 0.05 vs. Ctrl; ^#P < 0.05 between the marked groups. The significant difference was evaluated by one-way ANOVA followed by the post hoc Bonferroni test.