Research Paper Volume 15, Issue 19 pp 10705—10731
Targeting circular RNA-Glra2 alleviates retinal neurodegeneration induced by ocular hypertension
Figure 2. Validation of detection accuracy of circRNA microarray by qRT-PCR assays. qRT-PCR assays were conducted to compare the expression pattern of mmu_circ_0016226, mmu_circ_0016224, cicRNA.16113, cicRNA.7215, cicRNA.27992, and mmu_circ_0016436 between microbeads-injected retinas and saline-injected retinas. RNA samples were collected from microbeads-injected retinas (n = 12) and saline-injected retinas (n = 12) at 2-month after injection. RNA samples from 4 different retinas were pooled together as a biological replicate. Each group included 3 biological replicates.