Research Paper Volume 15, Issue 19 pp 10705—10731

Figure 10. cGlra2/miR-144/BCL2L11 signaling axis is involved in regulating RGC function. (A–C) RGCs were transfected with Scr siRNA, cGlra2 siRNA, scramble (Scr) mimics, miR-144 mimics, miR-144 mimics plus pcDNA3.1-BCL2L11, miR-144 mimics plus pcDNA3.1 (vector) or left untreated (Ctrl) for 12 h and then exposed to H₂O₂ (100 μmol/L) to mimic oxidative stress for additional 36 h. CCK-8 assays were performed to detect RGC viability (A, n = 4). Hoechst staining and quantification analysis was performed to detect the change of nuclei morphological characteristics of RGCs (B, n = 4, Scale bar: 50 μm). Caspase 3/7 activity was performed to detect the degree of RGC apoptosis (C, n = 4). ^*P < 0.05 vs. Ctrl group; ^#P < 0.05 between the marked group. All significance was examined using One-way ANOVA followed by Bonferroni’s post hoc test.