Research Paper Volume 15, Issue 19 pp 10681—10704
Analysis of potential biomarkers for diabetic kidney disease based on single-cell RNA-sequencing integrated with a single-cell sequencing assay for transposase-accessible chromatin
Figure 2. Comparative analysis of differentially expressed genes between db/db and db/m mice. (A) Functional enrichment analysis of the upregulated genes in the PTS3 cluster based on KEGG pathway analysis. The color corresponds to the significance values. (B) Heatmap showing the numbers of cell–cell interactions in the scRNA-seq datasets of db/m mice and db/db mice. The strength of cell–cell communication is indicated by color intensity. (C) Differential expression of Txnip and Wfdc2 in db/db and db/m mice. (D) Wfdc2 expression in the morning urine and kidneys of patients with diabetic kidney disease (DKD) and non-diabetic kidney disease (NDKD) controls, as analyzed by western blotting and immunohistochemistry. (E) Violin plots showing the expression of Klf10 and Klf11 in the kidneys of db/db and db/m mice.