Research Paper Volume 15, Issue 19 pp 9965—9983

Figure 3. One DNA content permits the survival of the CLS assay. (A) The number of cells with diploid and lower than 2c DNA content was observed at different time points during CLS of the diploid (BY4743) strain. (B) Number of cells with haploid and lower than 1c DNA content observed in different time points during CLS of haploid (BY4741) strain. (C) Comparison of survival rate of diploid (BY4743) versus haploid (BY4741) wild-type strains during CLS assay. The mean from three biological repetitions is shown. Bars indicate standard deviations. Statistical significance was assessed using ANOVA and Dunnett’s post hoc test (* p < 0.05; *** p < 0.001). (D) Number of cells with diploid, 1c, and lower than 1c DNA content observed in different time points during CLS of diploid (BY4743) strain. (A, C, D) The mean from three biological repetitions is shown. Bars indicate standard deviations. (E) Flow cytometry results show changes in the DNA content and cell size during the CLS experiment in the whole cell population and its fractions defined as diploids and cells with DNA contents lower than 2c or 1c. The upper panel shows scatter plots for exponentially grown haploid and diploid strains, which enabled the setting up of gating conditions to determine specific subpopulations.