Research Paper Volume 15, Issue 19 pp 10593—10606
UCHL1-PKM2 axis dysregulation is associated with promoted proliferation and invasiveness of urothelial bladder cancer cells
Figure 2. UCHL1 promotes the proliferation capacity of UBC cells in vitro. (A, B) WB and qRT-PCR tests on the UCHL1 protein and mRNA in several UBC cell lines including UM-UC-3, SW780, T24, J82, BIU-87 and 5637 (n = 3). (C, D) Design and WB/qRT-PRC functional validation assay of UCHL1-specific shRNAs and overexpression vectors (n = 3). (E–G) CCK8 proliferation assay to examine the impact of UCHL1 silencing or overexpression on the proliferative abilities of UBC cell lines including T24, J82 and UM-UC-3 (n = 3). (H, I) Colony formation assay to investigate the influences of UCHL1 silencing or overexpression on the proliferation of UBC cell lines (n = 3). (J, K) Ki67 and PCNA detection by WB and qRT-PCR method in UBC cell lines which were respectively treated by UCHL1-specific shRNAs or overexpression vectors (n = 3).