Research Paper Volume 15, Issue 19 pp 10540—10548
Sonic hedgehog signaling promotes angiogenesis of endothelial progenitor cells to improve pressure ulcers healing by PI3K/AKT/eNOS signaling
Figure 1. SHH pathway regulates PI3K/AKT/eNOS signaling in EPCs. (A) The EPCs were isolated from the mononuclear cells (MNCs). The DiI-acLDL and bind fluorescein iso-thiocyanate UEA-1 were analyzed by immunofluorescent analysis in the EPCs. Scale bar: 10 μm. (B) The EPCs were treated with SAG (1 μM) or cyclopamine (10 μM). (C) The patched-1, Gli-1, PI3K, Akt, eNOS expression and the Akt phosphorylation were tested by Western blot analysis in the EPCs. N = 3, mean ± SD, *p< 0.05, **p< 0.01.