Figure 6.Effects of repeated administration of Alp on learning and memory in mice. (A) Scheme of behavioral experiments. (B) The drinking error of each mouse was examined by ICS (n = 12). (C) The learning ability of each mouse was evaluated by NOR (n = 12). (D) HE staining in each region (CA1, CA2, CA3 and DG) of the hippocampus. (E) Counting of pyramidal neurons in each region (CA1, CA2, CA3 and DG) of the hippocampus (n = 6). (F) Immunohistochemical staining in each region (CA1, CA2, CA3 and DG) of the BDNF expression. (G) Statistical results of BDNF immunohistochemical in average optical density (n = 6). Scale bar is 100 μm. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01 vs. the control group; #p < 0.05, ##p < 0.01 vs. the Alp group.
