Research Paper Volume 15, Issue 19 pp 10407—10427

Figure 5. PLCL1 promotes autophagic flux by regulating the AMPK/mTOR signalling pathway in RCC cells. (A–C) Expression of LC3B and p62 in RCC cells with different treatments was examined using western blotting with quantitative analysis. GAPDH was used as a loading control. (D, E) 786-O and 769P cells transfected with GFP-mRFP-LC3B adenovirus were analysed using immunofluorescence. Autolysosome (red dots) and autophagosome (yellow dots) formation are shown using confocal microscopy and were quantitively analysed. Scale bar, 20 μm. (F–K) Representative western blotting and quantitative analysis of AMPK/mTOR signalling pathway-related proteins. Data are shown as the mean ± SE from three independent experiments. Student’s t test was performed to determine statistical significance between two groups. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001 versus vector or scramble group. ^#P < 0.05; ^#P < 0.01; ^#P < 0.001 versus cells overexpressing PLCLl or siRNA1.