Research Paper Volume 15, Issue 19 pp 10407—10427

Figure 4. PLCL1 facilitates autophagy in RCC cells. (A) The heatmap of clustering analysis according to the sequencing of differentially expressed genes. (B) KEGG pathway enrichment analysis of the top five results. (C–E) Transmission electron microscopy indicating the formation of autophagosomes or autolysosomes in RCC cells. Red arrows: autophagosomes or autolysosomes. Scale bar: 1 μm. (F) 786-O and ACHN cells transfected with PLCL1 lentivirus and vector were subjected to immunofluorescence and quantitative analysis for LC3B. Scale bar, 20 μm. (G) Representative confocal images and quantitative analysis of LC3 dots in 769P cells transfected with siRNA-scramble, siRNA-PLCL1 #1, and shRNA-PLCL1 #2. Scale bar, 20 μm. Data are shown as the mean ± SE from three independent experiments. Student’s t test was performed to determine statistical significance between two groups. ^*P < 0.05.