Research Paper Volume 15, Issue 19 pp 10407—10427

Figure 3. PLCL1 induces apoptosis in RCC cells. (A, B) Flow cytometry analysis was utilized to measure apoptosis in 786-O and ACHN cells transfected with PLCL1 or vector. (C, D) 769P cells were transfected with siRNA-PLCL1 #1, shRNA-PLCL1 #2 or siRNA-scrambled, and proliferation was examined using Edu kits, nuclei stained blue by DAPI, green for positive cells. Scale bar: 50 μm. (E–I) Representative western blots and quantification analysis of Bax and Bcl2 in RCC cells with different treatments. GAPDH was used as a loading control. Data are shown as the mean ± SE from three independent experiments. Student’s t test was performed to determine statistical significance between two groups. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.