Research Paper Volume 15, Issue 19 pp 10407—10427

Figure 1. PLCL1 expression is downregulated in RCC tumour tissues and closely related to RCC patient prognosis. (A) Differentially expressed genes (DEGs) in GSE36895, GSE53757, GSE68417, GSE66270, and GSE16441 were intersected using Venn diagrams. (B) Kaplan–Meier curve of RCC patients grouped based on median levels of PLCL1. HR, hazard ratio. (C) Univariate and multivariate analyses showing the relationship between significantly intersected DEG levels and RCC patient survival. CI, confidence interval. (D–F) Representative expression levels of PLCL1 in tumour samples and paired adjacent normal tissues (PANT) from public datasets. (G, H) Representative RT–qPCR and western blot analysis of PLCL1 in RCC tissue and PANT. (I) Quantification of PLCL1 protein levels. β-actin was used as a loading control. (J) Representative RT–qPCR analysis of PLCL1 in normal human kidney HK2 cells and tumour cells. (K, L) Representative western blots and quantification of PLCL1. GAPDH was used as a loading control. (M) Representative HE and immunohistochemistry analysis of PLCL1 in RCC tissue and PANT. Scale bar: 100 μm. Data are shown as the mean ± SE from three independent experiments. Student’s t test was performed to determine statistical significance between two groups. Scale bar: 100 μm. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.