Research Paper Volume 15, Issue 20 pp 11052—11066
The HNF4A-CHPF pathway promotes proliferation and invasion through interactions with MAD1L1 in glioma
Figure 4. Verify the mutual regulation relationship between CHPF and MAD1L1. (A) The proteins potentially interacted with CHPF by IP and LC-MS/MS assays. (B) Gene ontology analysis of potentially interacted proteins with CHPF. (C) The result of IP and LC-MS/MS assays. (D) CHPF expression was positively associated with MAD1L1 expression in GBM based on GEPIA dataset. (E) Effects of CHPF knockdown on inhibiting MAD1L1 expression in U251 and U87 cells. (F) Co-IP assays were conducted to verify the interaction between CHPF and MAD1L1 in U251 and U87 cells. (G) The interaction between CHPF and MAD1L1 was verified using GST pull-down assay. (H) Immunofluorescence experiment revealed the location of CHPF and MAD1L1 in 251 cells.