Research Paper Volume 15, Issue 20 pp 11052—11066
The HNF4A-CHPF pathway promotes proliferation and invasion through interactions with MAD1L1 in glioma
Figure 3. The significant role of CHPF in cell cycle of glioma and knock-down of CHPF inhibited glioma growth in vivo. (A) Effects of CHPF knockdown on cell cycle stages of U251 and U87 cells. (B) Effects of CHPF knockdown on the expression of cyclin A2, cyclin B1, cyclin E1, cyclin D1, CDK1, P21, P27 in U251 and U87 cells. (C, D) H&E staining of brain sections demonstrated a significant decrease in tumor volume after CHPF-knockdown at 30 days post implantation. (E) Kaplan-Meier survival curves showing a significant increase in median survival of CHPF-knockdown tumor-bearing mice. (F, G) The tissue micrographs of the two groups and the representative micrographs of the immunohistochemistry using antibodies against CHPF, Ki67, Cyclin B1 were shown. ns P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001.