Research Paper Volume 15, Issue 20 pp 11052—11066
The HNF4A-CHPF pathway promotes proliferation and invasion through interactions with MAD1L1 in glioma
Figure 2. Knock-down of CHPF inhibited glioma growth in vitro. (A) Western blot analysis showed CHPF expression in five glioma cell lines and human normal cells. (B, C) The knockdown efficiency was verified by WB and RT-qPCR assays in U251 and U87 cells. (D–F) Effects of CHPF knockdown on cell proliferation by CCK-8, EdU and colony formation assay in U251 and U87 cells. (G) Effects of CHPF knockdown on invasive capacities by transwell assay in U251 and U87 cells. (H) Effects of CHPF knockdown on migratory ability by wound healing assay in U251 and U87 cells. (I, J) Effects of CHPF knockdown on cell apoptosis via flow cytometry. ns P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001.