Figure 1.High glucose treatment induced oxidative stress and IR in MPC5 cells. (A) HG inducement distinctly reduced glucose consumption of MPC5 cells compared with normal glucose-treated group. (B) HG inducement notably augmented generations of malondialdehyde (MDA). (C–E) The phosphorylation of insulin receptor substrate (IRS)-1 (phospho Y632), AKT (phospho Ser473) and AKT (phospho Thr308) was markedly reduced by HG inducement. Besides, the expression of Nephrin was also notably decreased by HG inducement. (*P < 0.05; ** p < 0.01; *** p < 0.001, n=3).
